Plant Genome Editing with CRISPR-Cas9 and Cas12 Systems
Yiping Qi, Assistant Professor Dept. of Plant Science & Landscape Architecture
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 and Cas12a (CRISPR from Prevotella and Francisella 1, or Cas12a) are two leading genome editing nuclease systems for plant genome editing. In recent years, we have developed efficient and easy-to-use Cas9 and Cas12a toolkits, which have been used for plant genome editing by many researchers. Here, we will report successful examples of using these reagents to edit the genomes of Arabidopsis, rice, maize, tomato and carrot. To understand the scope of off-target mutations in Cas9 or Cas12a-edited crops, we recently used whole-genome sequencing (WGS) and CIRCLE-seq to assess off-target effects of these nucleases in rice and maize. Our comprehensive and rigorous analysis suggests both Cas9 and Cas12a nucleases are very specific in generating targeted DNA modifications in plants.
UPCOMING PSLA SEMINARS: http://psla.uconn.edu/SeminarsFall2019.pdf
For more information, contact: Yi Li or Huanzhong Wang at yi.li@uconn.edu or huanzhong.wang@uconn.edu