Presenter: Satoshi Yamazaki, Ph.D., Project Associate Professor, Project Division of Advanced Regenerative Medicine, The University of Tokyo, Japan
Title: “Optimizing in vivo generation of engraftable murine Hematopoietic stem cells from pluripotent stem cells through hemogenic endothelium in teratomas”
Abstract:Generation of hematopoietic stem cells (HSCs) from pluripotent stem cells (PSCs) could potentially provide unlimited HSCs for clinical transplantation, a curative treatment for numerous blood diseases. However, to date, bona fide HSC generation has been largely unsuccessful in vitro. We have previously described proof-of-concept for the in vivo HSC production from PSCs via teratoma formation. However, our first-generation system was complex and the output low. Here, we further optimize this technology and demonstrate: (1) simplified HSC generation using transcription factor overexpression; (2) improved HSC output using c-Kit-deficient host mice, and (3) that teratomas can be transplanted and cryopreserved. We demonstrate that overexpression ofGfi1b, c-Fos and Gata2, previously reported to transdifferentiate fibroblasts into hematopoietic progenitors in vitro, caninduce long-term HSC formation in vivo. Our in vivo system provides a useful platform to investigate new, and re-evaluate existing, strategies to generate HSCs and study early HSC development.
The Jackson Laboratory for Genomic Medicine Leo Holt Conference Room
1:00 pm
Host: Dr. Bill Skarnes
For more information, contact: Devin Davis at devin.davis@jax.org