ISG/MARS microbiome/amplicon processing workshop will run May 12-15 from 9:30 a.m. to 4:30 p.m. This four-day workshop is covers amplicon sequence processing from raw fastq through statistical analyses and generating publication quality figures.
This workshop will introduce users to two open-source command line tools: mothur and R. *No prior command line computer experience is assumed* We will cover how to quality control sequences including lectures to help participants understand the wet lab work that goes into generating the sequences. After qc of sequences we’ll cluster into OTUs and taxonomically ID each OTU. Once we’ve processed the sequences, we’ll move into an introduction to R and community ecology stats (multivariate, non-parametric stats that we use to analyze microbiome data). This workshop will focus on using mothur and R for reproducible research by learning statistical analyses and generating publication quality figures.
We’ll use some sample data that I provide throughout the workshop. Experience has taught me that trying to teach these tools with participants real data doesn’t work very well. However, both of these tools are command line which means that you will create a script with the series of commands and rerun them on your data by changing just a few words.
The course will be $570/participant ($786 for nonUConn participants) and is open to anyone, not just people that have had data generated through the center.
https://mars.uconn.edu/workshops/
For more information, contact: Kendra Maas at mars@uconn.edu