UConn PMF is excited to announce the fifth iteration of our annual hands-on proteomics workshop! This 3-day course will include both lectures and hands-on experience with sample preparation, instrumentation, and software designed to educate UConn scientists in current methodologies and analysis options for quantitative proteomics using state-of-the-art mass spectrometry (MS). The course will be co-taught by UConn PMF scientists Jeremy Balsbaugh, Ph.D., Jen Liddle, Ph.D., and Sonam Tamrakar, Ph.D.
When: 9.00am to 5.00pm, Wednesday March 19-21, 2025
Where: Pharmacy/Biology Building Rooms 131 (Lecture) and 511/513 (Lab), UConn Storrs Campus
Cost: $440/person (Internal UConn/UConn Health rate) and subject to change based on final total reagent/experimental costs. Inquire about external academic institution rates if applicable.
What I will learn: The full agenda will be announced in the coming weeks, but intended topics to be covered will include:
- Introduction to ultra-high performance liquid chromatography
- Introduction to high resolution tandem MS
- Label-free and label-based methods for quantitative MS-based proteomics
- Data-Dependent vs Data-Independent Acquisition (DDA/DIA) MS/MS methods
- Bioinformatics analysis and options currently available to UConn/UConn Health researchers
- Proteomics sample preparation
- In-gel digestions
- In-solution digestions (e.g. Co-immunoprecipitations, whole cell lysates)
- Applications of MS-proteomics outside of standard protein identification/quantitation
- Data analysis tutorials for our in-house analytic platform in R (msDiaLogue) featuring the UConn Statistical Consulting Services group and SCS Director, Timothy Moore, Ph.D.
Admission to the workshop will be strictly limited due to space restrictions and access to instrumentation. Attendance will be evenly split between UConn Storrs and UConn Health researchers to allow equal participation between both campuses. In addition, a restriction of one attendee per laboratory may be instituted to provide access for a wider range of participants across departments and research groups. A waitlist will be created once the number of interested attendees surpasses the initial 20 attendee slots.
Soon after successful registration, you will receive confirmation directly from PMF as to your standing on either the attendance list or waitlist. Any non-UConn scientists will be added to the attendance list only if slots remain (registration fee may vary for external attendees). To save your spot, fill out the registration form posted at https://proteomics.uconn.edu/workshops/
Participation will be granted on a first come, first served basis for registered attendees.
Jeremy L. Balsbaugh, Ph.D.
Director, UConn Proteomics & Metabolomics Facility (PMF)
Director, Center for Open Research Resources & Equipment (COR²E)
Office of the Vice President for Research
For more information, contact: Jeremy Balsbaugh at jeremy.balsbaugh@uconn.edu