The MCB PSM program in Microbial Systems Analysis is offering two advanced modules in May term 2019, open to graduate students and qualified undergraduate students. The modules are an excellent opportunity to learn more advanced skills that are now commonly used in research and industry.
MCB 5670-02 Theory and Practice of Laboratory Techniques in Microbiology – Bacterial DNA & RNA Isolation and Quality Control
Students use different methodologies for DNA/RNA isolation and quality control. Emphasis will be placed on comparing the different techniques and instruments. Students will isolate DNA and RNA using different kits and lab protocols. Quantification and quality assessment will be done using a Nanodrop, Qubit (fluorometer), Bioanalyzer 2100, and TapeStation 4150. If interested, students can bring their own samples.
MCB 5671-02 Advanced Theory and Practice of Laboratory Techniques in Microbiology – Characterization of Microbial Communities by 16S rRNA gene sequencing
In this module, students will learn the principles of NextGeneration Sequencing using an Illumina MiSeq and microbiome analyses. Each student will sequence the V4 region of the 16S rRNA genes of a microbial community. Students will learn how to prepare the libraries for running on the MiSeq and analyze the data after the run is completed. The data analysis involves a QIIME pipeline that is widely used to analyze microbiome datasets.
More information about the modules can be found here. Prerequisite is MCB 5427 Introduction to Molecular Biology Techniques or permission of instructor. Please contact elaine.mirkin@uconn.edu for permission number. Information on registration and payment deadlines can be found on the Summer Session 2019 website along with cost information. Graduate tuition and fees apply; graduate assistantship tuition waivers do not cover May term and summer session tuition and fees.
For more information, contact: Elaine Mirkin at elaine.mirkin@uconn.edu
For more information, contact: Elaine Mirkin at elaine.mirkin@uconn.edu